A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
نویسندگان
چکیده
منابع مشابه
A New Epoxy Embedment for Electron Microscopy
A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Ep...
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A new epoxy embedding mixture for biological material recently has been introduced by Freeman and Spurlock (1). This mixture consists of Mara-glas 655, Cardolite NC-513, dibutyl phthalate, and the curing agent benzyldimethylamine (BDMA). Maraglas has certain advantages over other resins and epoxies, notably, wide range of miscibility, low viscosity, ease of sectioning, good staining qualities, ...
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A method of embedding biological specimens in araldite 502 (Ciba) has been developed for materials available in the United States. Araldite-embedded tissues are suitable for electron microscopy, but the cutting qualities of the resin necessitates more than routine attention during microtomy. The rather high viscosity of araldite 502 also seems to be an unnecessary handicap. The less viscous epo...
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During the early phases of tissue sectioning for electron microscopy, virtually all of the standard cytological fixatives were tried. Of these, osmium tetroxide appeared to give the best preservation, and this was further improved by buffering the fixative solution at a slightly alkaline pH (Palade (1)). A variant developed by Dalton (2) contains Os04 in a chromate-dichromate system. Formalin h...
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A new water-soluble embedding agent, tr ial p roduc t CIBA X 133/20971 has recently been in t roduced for selected electron microscopic investigations (1). D u r c u p a n subsequently proved to be miscible wi th the water-insoluble epoxy resin Araldi te 2 which is commonly used for tissue embedding (2). In this way, an imal tissues can be embedded in Araldi te wi thout being b rough t into con...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1962
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.13.3.437